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Evidence derived from human observational studies, autopsy findings, and experimental models suggests that sustained androgen receptor overstimulation may induce structural, functional, and metabolic alterations that compromise cardiovascular homeostasis ( Cardiovascular pathology represents one of the most clinically relevant consequences of AAS misuse

The skilled artisan could, using procedures well-known in the art, obtain the polynucleotide sequence corresponding to full-length genes (including, but not limited to the full-length coding region), allelic variants, splice variants, orthologs, and/or species homologues of genes corresponding to SEQ ID NO:1, or a deposited clone, relying on the sequence from the sequences disclosed herein or the clones deposited with the ATCC

Next, the supernatant was collected after centrifuging at 1000 rpm for 10 min at 4 C, and then measured at 532 nm with a microplate reader

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