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In vitro epithelial infection model For our infections, the medium was removed and replaced with prewarmed fresh RPMI-1640 (or RPMI-1640 with L-glutamine but without glucose, catalog number 11879020, Thermo Fisher Scientific) without FBS, and supplemented with or without 10 mg/mL albumin

A safety-first approach matters because: Supplements arent regulated the same way as prescription medications, and dosages/quality can vary

It is produced by the gut bacterium Blautia producta and drives macrophage priming and hepatic stellate cell activation by activating the GPR119/TAK1/NF-B/TGF-1 signaling pathway, thereby promoting inflammation and fibrosis

Above all, tumor-specific CTLs in the bloodstream cannot infiltrate into the TME due to this abnormal tumor vasculature and, as a result, are not able to eradicate tumor cells