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doi: 10.12688/f1000research.20906.1 139

Next, in all cases, the liposome/protein mixture (final volume 150 L) was adjusted to 28% (w/w) sucrose by mixing 100 L of a 60% (w/w) sucrose solution in HK buffer and overlaid with 200 L of HK buffer containing 24% (w/w) sucrose and 50 L of sucrose-free HK buffer

Statistical analysis was performed using either an unpaired, two-tailed t -test, paired t -test or an ordinary one-way or two-way ANOVA with Dunnetts, dks or Tukeys multiple comparison correction