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Furthermore, the relative abundance of Akkermansia , a genus positively associated with improvements in obesity-related metabolic issues 60 , was increased in the vehicle-treated group that underwent diet switch relative to the WD control group, suggesting a potential for distinct microbiome-mediated mechanisms of weight loss subsequent to diet switch from those of diet switch in combination with 5A-1MQ treatment

The method of claim 1, wherein the set of mutagenic oligonucleotides comprises a 19-fold degenerate mutagenic oligonucleotide for each codon to be mutagenized, wherein each of the 19-fold degenerate mutagenic oligonucleotides comprises a homologous first sequence and a degenerate triplet second sequence

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Restore the Gut and the Estrobolome Healing the gut barrier and rebuilding microbial diversity is foundational