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To test the sensitivity of yeast to gliotoxin, BY4741 harboring either vector alone (pRS315) or pC-GliT was grown to mid-exponential phase (310 6 cells/ml)

In a preferred embodiment, the GSH transporter mediates at least one of endocytosis and transcytosis, the ligand and the pharmaceutical acceptable carrier is selected to bypass lysosomal degradation of the agent in the cell