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In a more complex experiment with a larger sample size and less clean-cut results, it's important to verbalize your null and alternative hypotheses so that you can't trick yourself -- or anyone else -- into believing you were actually testing something else

Two distinct quisqualate receptors regulate Ca 2+ homeostasis in hippocampal neurons in vitro

In addition, discordant (oppositive) differential mRNA expression of genes associated to the IGF-1 signaling pathway has been observed in kidney samples from mice undergoing post-ischemic renal repair, compared to gene expression profiles reported in human renal cell carcinoma

This can be a sign the treatment is working