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A peptide left at room temperature overnight isn't 'less effective,' it's non-functional

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After thawing, 2.5 10 5 cells were seeded in 0.5 ml of cell plating medium (CP medium, BioIVT) per well in collagen-coated 24-well plates and cultured for 4 h at 37 C in a humidified atmosphere with 5% CO 2