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For example, the Lactobacillus rhamnosus GG strain may modulate intestinal immune cells to reduce the release of inflammatory cytokines (Li et al., 2020), whereas Bifidobacterium longum NCC3001 may exert direct effects on the central nervous system via the vagus nerve to enhance the synthesis of neurotransmitters such as -aminobutyric acid ( 4.3 The role of B vitamins and vitamin D In children with ASD, abnormalities in methylation metabolism have been widely documented, including a decreased S-adenosylmethionine to S-adenosylhomocysteine (SAM/SAH) ratio and elevated homocysteine levels (Wilson et al., 2021)