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Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)

Tissue specificity and sharing of the microbiome regulation To investigate the tissue specificity of the microbiome regulation by gene expression, we performed an intersection of the gene-to-microbiome regulatory pairs across the three intestinal tissues (Fig

To calculate absolute (i.e., water-referenced) GSH levels from GSH/Cr ratios, concentrations of total Cr (Cr plus phosphocreatine) were calculated

College of Liberal Arts & Sciences Charlie Wichita, La Vista, Nebraska