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Escherichia coli strains were grown at 37C shaken at 170 rpm in liquid LuriaBertani (LB) broth or on solid LB medium, and ampicillin (100 g/ml), kanamycin (100 g/ml), streptomycin (100 g/ml), or chloromycetin (34 g/ml) was added when required

Overactivation of the ECS in Obesity Obesity and coexisting disorders such as insulin resistance, hypertension, and hypertriglyceridemia lead to the development of metabolic syndrome and type 2 diabetes (75)

This disparity underscores a critical knowledge gap that must be resolved before confident, evidence-based clinical adoption can be justified

This alteration in the gut microbiota composition will then lead to dysbiosis (gut imbalance) and inflammation which then compromises the gut and brain connection