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The results revealed that NGF administration significantly improved the survival of the transplanted cells, increased the DA content in local brain tissue, and led to a more significant improvement in motor function compared to cell transplantation [82]

Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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