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In addition, the vaccine improved the physical activity of middle-aged mice (50 weeks old) and extended the median lifespan of ZMPSTE24 KO mouse (model of progeria), an extreme model of accelerated aging 62

F.WhaleyR.et al (2021)

Pain behavior was quantified periodically at 5- and 35- min intervals (representative values of phases 1 and 2) by counting the number of flinches exhibited by the injected paw after injection